Welcome
to the Drosophila Sheffield
RNAi Screening Facility (SRSF). Based at the University of Sheffield, we provide a service for RNAi screening experiments in Drosophila cells.
Our service supplies to the wider scientific community whole genome screens at low cost. We offer either pre-aliquoted whole genome RNAi libraries or sub-libraries of target probes.
We support both plate reader assays, high-content microscopy as well as the equipment needed to process these samples in a high-throughput fashion. This can either be used to identify genes involved in disease representing future drug targets, or to identify genes involved in biological processes.
The major role of our staff is to generate the libraries and to assist screeners through the process before they carry out their experiments at the facility.
The screening process can be split into a number of discrete steps and organised in two stages between your institute and the SRSF.
Assays are usually developed in the screeners laboratory before discussion with our staff and then followed by the running of an assay development plate.
We provide expert help and assistance, including assay development and optimization, data and image analysis, and planning of follow-up assays and secondary hit determination.
In the first instance, contact us to discuss your screening requirements and we will advise you on the options available.
We offer genome-wide RNAi screens, a flexible service, including bespoke library construction and use of our equipment.
Once your requirements have been discussed, the screening process requires a number of forms to be completed before work can commence. When the assay has been developed you will be placed in queue ready to screen.
Latest News
RNAi in Drosophila Cells
Drosophila cells are the ideal tool for genome-wide screens because they represent a low complexity, low cost system amenable to RNAi screening.
Contact us
Please feel free to contact us about our screening service. Our facility is based at the department of Biomedical Science at the University of Sheffield, UK. We are open to applications from both UK and overseas research institutes.
- Tel: 0114 222 3690
- Email: rnai@sheffield.ac.uk
- Fill in our Enquiry form
The Sheffield RNAi Facility is funded by the Wellcome Trust
